mouse tgf β1  (Cell Signaling Technology Inc)

Journal: Febs Letters

Article Title: Transcriptional regulation of basophil‐specific protease genes by C/ EBPα , GATA2 , TGF ‐β signaling, and epigenetic mechanisms

doi: 10.1002/1873-3468.15069

Figure Lengend Snippet: Roles of C/EBPα in the expression of MC/basophil‐related transcription factors and effects of the TGF‐β stimulation on the expression of basophil‐specific proteases and related transcription factors. (A, B) Messenger RNA levels of transcription factors in Cebpa siRNA‐transfected (A) and Cebpa ‐overexpressing (B) BM cells. (C) Effects of the TGF‐β treatment on BM cells cultured for 7 weeks. The results shown in figure are the mean ± SE of data obtained by 7 (A), 4 (B), and 3 (C) independent experiments. Significance was assessed by the two‐tailed unpaired Welch's t ‐test (A–C). * P < 0.05; ** P < 0.01.

Article Snippet: DAPI (11034‐56; Nacalai Tesque, Kyoto, Japan), DMSO (07‐4860‐5; Sigma Aldrich, St. Louis, MO, USA), 5‐aza‐2′‐deoxycitidine (A2232; Tokyo Chemical Industry, Tokyo, Japan), trichostatin A (TSA) (T6933; Fujifilm Wako Pure Chemical, Osaka, Japan), Fc block (clone 93, BioLegend or clone 2.4G2; Tonbo Biosciences, San Diego, CA, USA), and recombinant mouse TGF‐β (5231; Cell Signaling Technology, Danvers, MA) were purchased from the indicated sources.

Techniques: Expressing, Transfection, Cell Culture, Two Tailed Test

Journal: iScience

Article Title: Kidney fibrosis molecular mechanisms Spp1 influences fibroblast activity through transforming growth factor beta smad signaling

doi: 10.1016/j.isci.2024.109839

Figure Lengend Snippet:

Article Snippet: TGF-β1 , Cell Signaling, USA , 5231LF.

Techniques: Recombinant, Staining, CCK-8 Assay, Enzyme-linked Immunosorbent Assay